Protein_Domain

Part:BBa_K4803001

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)

gp80(IL6Rα) extracellular domain

Usage and Biology

Glycoprotein 80 (gp80), also known as interleukin 6 receptor-alpha (IL6R-alpha), is a type of cytokine receptor that acts in mammalian cells. This protein is expressed in the human body and forms a gp80-IL6-gp130 (IL6R-beta) trimer in the presence of IL6. The trimer also binds to each other to form a hexamer. This hexamer formation reaction mediates cytokine signaling by causing activation of associated cytosolic tyrosine kinases and subsequent modification of transcription factors[1].

control
Figure 1. IL6 signaling
The cDNA encoding gp80 from [2] which is the extracellular domain was optimized with IDT codon optimization tool to generate this part. In UTokyo2023 project, this part was used as part of the MESA system [3], in which gp80 forms homodimers or heterodimers with gp130(BBa_K4803000) in the presence of IL6, which in turn acts on proteases in the cytoplasm to release protein of interest into the cytosol.
control
Figure 2. Overview of MESA system

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 166
    Illegal NgoMIV site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Kaur, S., Bansal, Y., Kumar, R., & Bansal, G. (2020). A panoramic review of IL-6: Structure, pathophysiological roles and inhibitors. Bioorganic & medicinal chemistry, 28(5), 115327. https://doi.org/10.1016/j.bmc.2020.115327

[2] Rodrigues, F. B. B., da Silva, R., Santos, E. F. D., de Brito, Mioni Thieli Figueiredo Magalhães, da Silva, Andréa Luciana Soares, de Meira Leite, M., Póvoa da Costa, F., de Nazaré do Socorro de Almeida Viana, Maria, de Sarges, Kevin Matheus Lima, Cantanhede, M. H. D., Veríssimo, Adriana de Oliveira Lameira, Carvalho, M. d. S., Henriques, D. F., Silva, C. P. d., Costa, I. B., Nunes, J. A. L., Costa, I. B., Viana, G. M. R., Queiroz, M. A. F., . . . Santos, Eduardo José Melo Dos. (2023). Association of polymorphisms of IL-6 pathway genes (IL6, IL6R and IL6ST) with COVID-19 severity in an amazonian population. Viruses, 15(5), 1197. https://doi.org/10.3390/v15051197

[3] Daringer, N. M., Dudek, R. M., Schwarz, K. A., & Leonard, J. N. (2014). Modular extracellular sensor architecture for engineering mammalian cell-based devices. ACS synthetic biology, 3(12), 892-902. https://doi.org/10.1021/sb400128g


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